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genetically-engineered expression construct, phtlv-1 gag-eyfp  (GenScript corporation)

 
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    Structured Review

    GenScript corporation genetically-engineered expression construct, phtlv-1 gag-eyfp
    Genetically Engineered Expression Construct, Phtlv 1 Gag Eyfp, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genetically-engineered+expression+construct%2C+phtlv-1+gag-eyfp/genetically+engineered+expression+construct++phtlv+1+gag+eyfp/pmc03951590-329-9-34
    Average 90 stars, based on 1 article reviews
    genetically-engineered expression construct, phtlv-1 gag-eyfp - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Interrelationship Between Cytoplasmic Retroviral Gag Concentration and Gag-Membrane Association
    Article Snippet: .. Briefly, this model system utilizes a genetically-engineered expression construct, pHTLV-1 Gag-EYFP, with the CMV promoter driving expression of a human codon-optimized HTLV-1 gag gene – designed using the UpGene program 69 and synthesized by GenScript Co. (Piscataway, NJ) – containing an optimal Kozak consensus sequence 70 ; 71 that was derived from the vector, pEYFP-N3 72 . .. Site-directed mutant derivatives of HIV-1 Gag-EYFP 72 and HTLV-1 Gag-EYFP, i.e., G2A Gag-EYFP and MA-EYFP derivatives, were made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla CA) per manufacturer’s instructions.

    Construct:

    Article Title: Interrelationship Between Cytoplasmic Retroviral Gag Concentration and Gag-Membrane Association
    Article Snippet: .. Briefly, this model system utilizes a genetically-engineered expression construct, pHTLV-1 Gag-EYFP, with the CMV promoter driving expression of a human codon-optimized HTLV-1 gag gene – designed using the UpGene program 69 and synthesized by GenScript Co. (Piscataway, NJ) – containing an optimal Kozak consensus sequence 70 ; 71 that was derived from the vector, pEYFP-N3 72 . .. Site-directed mutant derivatives of HIV-1 Gag-EYFP 72 and HTLV-1 Gag-EYFP, i.e., G2A Gag-EYFP and MA-EYFP derivatives, were made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla CA) per manufacturer’s instructions.

    Synthesized:

    Article Title: Interrelationship Between Cytoplasmic Retroviral Gag Concentration and Gag-Membrane Association
    Article Snippet: .. Briefly, this model system utilizes a genetically-engineered expression construct, pHTLV-1 Gag-EYFP, with the CMV promoter driving expression of a human codon-optimized HTLV-1 gag gene – designed using the UpGene program 69 and synthesized by GenScript Co. (Piscataway, NJ) – containing an optimal Kozak consensus sequence 70 ; 71 that was derived from the vector, pEYFP-N3 72 . .. Site-directed mutant derivatives of HIV-1 Gag-EYFP 72 and HTLV-1 Gag-EYFP, i.e., G2A Gag-EYFP and MA-EYFP derivatives, were made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla CA) per manufacturer’s instructions.

    Sequencing:

    Article Title: Interrelationship Between Cytoplasmic Retroviral Gag Concentration and Gag-Membrane Association
    Article Snippet: .. Briefly, this model system utilizes a genetically-engineered expression construct, pHTLV-1 Gag-EYFP, with the CMV promoter driving expression of a human codon-optimized HTLV-1 gag gene – designed using the UpGene program 69 and synthesized by GenScript Co. (Piscataway, NJ) – containing an optimal Kozak consensus sequence 70 ; 71 that was derived from the vector, pEYFP-N3 72 . .. Site-directed mutant derivatives of HIV-1 Gag-EYFP 72 and HTLV-1 Gag-EYFP, i.e., G2A Gag-EYFP and MA-EYFP derivatives, were made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla CA) per manufacturer’s instructions.

    Derivative Assay:

    Article Title: Interrelationship Between Cytoplasmic Retroviral Gag Concentration and Gag-Membrane Association
    Article Snippet: .. Briefly, this model system utilizes a genetically-engineered expression construct, pHTLV-1 Gag-EYFP, with the CMV promoter driving expression of a human codon-optimized HTLV-1 gag gene – designed using the UpGene program 69 and synthesized by GenScript Co. (Piscataway, NJ) – containing an optimal Kozak consensus sequence 70 ; 71 that was derived from the vector, pEYFP-N3 72 . .. Site-directed mutant derivatives of HIV-1 Gag-EYFP 72 and HTLV-1 Gag-EYFP, i.e., G2A Gag-EYFP and MA-EYFP derivatives, were made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla CA) per manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Interrelationship Between Cytoplasmic Retroviral Gag Concentration and Gag-Membrane Association
    Article Snippet: .. Briefly, this model system utilizes a genetically-engineered expression construct, pHTLV-1 Gag-EYFP, with the CMV promoter driving expression of a human codon-optimized HTLV-1 gag gene – designed using the UpGene program 69 and synthesized by GenScript Co. (Piscataway, NJ) – containing an optimal Kozak consensus sequence 70 ; 71 that was derived from the vector, pEYFP-N3 72 . .. Site-directed mutant derivatives of HIV-1 Gag-EYFP 72 and HTLV-1 Gag-EYFP, i.e., G2A Gag-EYFP and MA-EYFP derivatives, were made using the QuikChange XL Site-Directed Mutagenesis Kit (Stratagene, La Jolla CA) per manufacturer’s instructions.



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